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KMID : 0665220040170030294
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Korean Journal of Food and Nutrition
2004 Volume.17 No. 3 p.294 ~ p.301
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Effects of Various Proteins on the Autoxidation of L-Ascorbic Acid
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Kim Mi-Ok
Jang Sang-Moon
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Abstract
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Effects of superoxide dismutase(SOD), catalase(CAT), and such other proteins as bovine serum albumin(BSA), ovalbumin, lysozyme, and v-globulin on the autoxidation rates of L-ascorbic acid(AsA) in the absence of heavy metal ions and in the presence of Fe(III) or Cu(II) ions in water were examined. AsA was dissolved in a ultra-refined water at a concentration of 50 {mu}M and 5 {mu}M Fe(III) or 0.1 {mu}M Cu(II) were added, and a oxygen gas was bubbled through the solution at a flow rate of 200 ml/min at 35^{circ}C. The amount of remaining AsA in the reaction mixture was determined by using a UV spectrophotometer(at 265 nm). It was found that the Cu(II) at a concentration of 0.1 {mu}M had a more accelerated for the autoxidation of AsA than Fe(III) at 5 {mu}M. Moreover, it was confirmed that the ratio of remaining AsA was significantly larger in the presence of SOD, CAT, BSA, ovalbumin, lysozyme, and v-globulin than in the absence of proteins. The stabilization of AsA by various proteins were confirmed during the autoxidation of AsA in the presence of Fe(III) or Cu(II) in water. It was suggested that the non-enzymatic effects of SOD, CAT and some other proteins might be involves in the stabilization of AsA.
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KEYWORD
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L-ascorbic acid, autoxidation, protein, heavy metal ions
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